Extiquick™ cfDNA Extraction Kit (1-5ml plasma/serum)
Product Introduction
The Extiquick™ cfDNA Extraction Kit uses highly efficient, superparamagnetic, hydroxyl-modified, uniform-sized magnetic beads and a specially formulated buffer system to quickly and efficiently extract genomic DNA-free cfDNA from 1-5 mL of plasma, serum, or urine. This kit is easy to use, has a short workflow, yields high-quality cfDNA with high purity and consistent quality, is safe and reliable, and is especially suitable for use with automated extraction workstations. It is applicable to qPCR, next-generation sequencing, and other applications.
Product Advantages
High yield and purity of extracted nucleic acids.
No need to adjust buffer volume to meet the extraction needs of 1-5 ml samples.
Compatible with open automated extraction instruments; no specialized equipment is required.
Product Advantages


Figure 1: Comparison of cfDNA extraction using Extiquick and TG cfDNA extraction kits on eight 2 mL samples, showing the total amount of extracted cfDNA.

Figure 2: Fragmentation analysis of the eight extracted plasma samples using QSEP. Extiquick (left) shows normal fragments with no genomic contamination, while TG (right) shows minor genomic contamination in some samples.

Figure 3: The Extiquick™ cfDNA Extraction Kit shows a linear distribution of cfDNA yield from different plasma volumes of various samples without changing buffer or magnetic bead volume.

Figure 4: Extraction of plasma from 120 tumor samples shows that this kit can consistently extract more than 40 ng of total cfDNA from 2 mL of plasma.

Figure 5: After library construction of 2 mL of cfDNA using the XLIBPreP™ Enzymetics DNA Library Kit and sequencing on the NovaSeq 6000 platform, analysis of insert fragment size shows cfDNA insert fragment sizes of ~40 bp, ~70 bp, ~160 bp, and ~320 bp. This kit has high recovery efficiency for small fragments.
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