First-generation sequencing platform

Application of first-generation sequencing platforms in hematological diseases • Fragment analysis: A highly sensitive and high-resolution electrophoresis technique. Fluorescently labeled primers are used to PCR amplify the target nucleic acid. The size and conformation of the amplified product are analyzed by electrophoresis. • Nucleic acid variation: Nucleic acid fragments of specific sizes are used to detect variations such as SNPs and insertion/deletion mutations. This is considered the gold standard.

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Consumables

KIRGEN uses the finest raw materials and the most advanced processes, pursuing excellence in quality. Strictly adhering to FDA's QSR standards, the products are free of any odor, with minimal wall residue to ensure they are free of DNase, RNase, and pyrogens.

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Covaris ultrasonic fragmentation

Adaptive Focused Acoustics (AFA) technology; uses an isothermal, non-contact method for acoustic homogenization and acoustic lysis of samples.

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Ultrasonic disruption Qubite® 4.0 fluorometer

Powerful dual-core processor, fast and accurate quantification of DNA, RNA, and protein within 5 seconds; high accuracy, minimum detection of 1 μL sample (10 pg/μL DNA or 12.5 μg/mL protein); high specificity: selective quantification, distinguishing dsDNA, Oligos, RNA, MicroRNA, or Protein.

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Fast endonuclease

LightNing rapid restriction enzymes are a class of rapidly acting restriction enzymes that have been genetically engineered. All SwiftCut series enzymes can complete the specific cleavage of DNA within 5-15 minutes, suitable for rapid digestion of plasmid DNA, PCR products, and genomic DNA. All SwiftCut series enzymes have 100% activity in the common 10 × SwiftCut Buffer or 10 × SwiftCut Color Buffer, making it easy to perform multiple digestion reactions simultaneously in the same reaction system.

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Luciferase substrate and detection

D-Luciferin is a commonly used substrate for luciferase, widely used in the biotechnology field, especially in in vivo bioluminescence imaging. Under the action of ATP and luciferase, luciferin can be oxidized and its chemiluminescence detected at 560 nm. Luciferin is encoded by the luc gene, which exists as a reporter gene in various cells.

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Nucleic acid electrophoresis

Ready-to-use instant granule series products, based on the combination of biology and pharmaceuticals, have achieved large-scale, fully automated production processes through technological innovation and upgrading of production processes. This improves product stability and ensures experimental reproducibility. Its application in routine experiments frees experimenters from the constraints of balances, weighing paper, and PH meters, saving time and enabling efficient experimental operations.

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Plasmid transformation reagent

Used for plasmid transformation, gene cloning, and blue-white screening experiments. Suitable for efficient transformation of PCR products, cDNA, and other sources of unmethylated DNA.

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